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goat polyclonal human trf2  (Novus Biologicals)


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    Novus Biologicals goat polyclonal human trf2
    FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat <t>polyclonal</t> antibody against PML and a rabbit polyclonal antibody (FC-08) against <t>TRF2.</t> (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.
    Goat Polyclonal Human Trf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+human+trf2/Human+TRF2+Antibody/10__1128_slash_mcb__24__13__5967___5977__2004-105-13-17
    Average 90 stars, based on 4 article reviews
    goat polyclonal human trf2 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance"

    Article Title: p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance

    Journal: Molecular and Cellular Biology

    doi: 10.1128/mcb.24.13.5967-5977.2004

    FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat polyclonal antibody against PML and a rabbit polyclonal antibody (FC-08) against TRF2. (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.
    Figure Legend Snippet: FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat polyclonal antibody against PML and a rabbit polyclonal antibody (FC-08) against TRF2. (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.

    Techniques Used: Expressing, Cell Culture, Generated, Quantitation Assay, BrdU Incorporation Assay, BrdU Staining, Staining

    FIG. 4. ChIP analysis. (A) Telomeric DNA is precipitated by anti- bodies against the telomeric binding proteins TRF2 but not by non- specific goat IgG, irrespective of culture conditions. Immunoprecipi- tation requires formaldehyde cross-linking of proteins to DNA. p53 is also associated with telomeric DNA in the HIO107-TA cell line but only at the permissive temperature of 32°C. Antibodies against TRF2 do not immunoprecipitate centromeric -satellite sequences. (B) Quantitation of the data shown in panel A. Percent input precip- itated (ppted) [(signal strength of telomeric DNA precipitated with the indicated specific antibody background signal obtained with nonspecific IgG)/input signal] 100. (C) Agarose gel of PCR products using primers to amplify the p53 binding site at position 1.4 kb within the p21WAF1 promoter. The template DNAs are identical to those interrogated for the experiment shown in panel C. The predicted product of 113 bp is only detected in DNA from the ChIP assay carried out with an antibody against p53 at 32°C. MW, PhiX HaeIII markers. Antibodies used for all panels are as follows: Gt TRF2, goat polyclonal antibody against TRF2 (Imgenex); Gt p53, goat polyclonal antibody against the carboxy terminus of human p53 (C-19; Santa Cruz); Gt IgG, goat IgG (Santa Cruz).
    Figure Legend Snippet: FIG. 4. ChIP analysis. (A) Telomeric DNA is precipitated by anti- bodies against the telomeric binding proteins TRF2 but not by non- specific goat IgG, irrespective of culture conditions. Immunoprecipi- tation requires formaldehyde cross-linking of proteins to DNA. p53 is also associated with telomeric DNA in the HIO107-TA cell line but only at the permissive temperature of 32°C. Antibodies against TRF2 do not immunoprecipitate centromeric -satellite sequences. (B) Quantitation of the data shown in panel A. Percent input precip- itated (ppted) [(signal strength of telomeric DNA precipitated with the indicated specific antibody background signal obtained with nonspecific IgG)/input signal] 100. (C) Agarose gel of PCR products using primers to amplify the p53 binding site at position 1.4 kb within the p21WAF1 promoter. The template DNAs are identical to those interrogated for the experiment shown in panel C. The predicted product of 113 bp is only detected in DNA from the ChIP assay carried out with an antibody against p53 at 32°C. MW, PhiX HaeIII markers. Antibodies used for all panels are as follows: Gt TRF2, goat polyclonal antibody against TRF2 (Imgenex); Gt p53, goat polyclonal antibody against the carboxy terminus of human p53 (C-19; Santa Cruz); Gt IgG, goat IgG (Santa Cruz).

    Techniques Used: Binding Assay, Quantitation Assay, Agarose Gel Electrophoresis

    Related Articles

    Incubation:

    Article Title: p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance
    Article Snippet: .. The precleared chromatin was incubated overnight at 4°C with the following primary antibodies: goat polyclonal human TRF2 (Imgenex), goat polyclonal p53 (C-19; Santa Cruz), or goat serum IgG (Santa Cruz). ..

    Article Title: p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance
    Article Snippet: .. The precleared chromatin was incubated overnight at 4°C with the following primary antibodies: goat polyclonal human TRF2 (Imgenex), goat polyclonal p53 (C-19; Santa Cruz), or goat serum IgG (Santa Cruz). ..



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    goat polyclonal human trf2  (Novus Biologicals)
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    Novus Biologicals goat polyclonal human trf2
    FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat <t>polyclonal</t> antibody against PML and a rabbit polyclonal antibody (FC-08) against <t>TRF2.</t> (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.
    Goat Polyclonal Human Trf2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+human+trf2/Human+TRF2+Antibody/10__1128_slash_mcb__24__13__5967___5977__2004-105-13-17
    Average 90 stars, based on 1 article reviews
    goat polyclonal human trf2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat polyclonal antibody against PML and a rabbit polyclonal antibody (FC-08) against TRF2. (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.

    Journal: Molecular and Cellular Biology

    Article Title: p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance

    doi: 10.1128/mcb.24.13.5967-5977.2004

    Figure Lengend Snippet: FIG. 3. Expression of ts-p53TA perturbs APB frequency and causes an S phase delay in HIO107 ALT cells. (A) The frequency of APB-positive cells is increased two- to threefold when cell lines expressing either ts-p53WT or ts-p53TA are cultured at 32°C. APBs were detected by costaining the cells with a goat polyclonal antibody against PML and a rabbit polyclonal antibody (FC-08) against TRF2. (B) The HIO107-TA ALT cell line incorporates BrdU, but the cells accumulate in early S phase after 72 h at 32°C, while the HIO114 cell line is unaffected by growth at 32°C. The left panel shows a FACS analysis of BrdU intensity (y axis) versus DNA content (x axis). The right panel shows the cell cycle profiles generated from the data on the left of the figure (y axis, number of events; x axis, DNA content). (C) The parental HIO107 and the HIO107-TA cell lines were arrested by exposure to HU and, following release, cultured at either 32 or 39°C for the indicated time prior to harvest. The presence of active ts-p53TA prevents cells from progressing through S phase following the removal of HU. y axis, number of events; x axis, DNA content. (D) Quantitation of the cell cycle distribution of the populations shown in panel C. HIO107-TA cells accumulate in S phase for up to 24 h, suggesting that S phase is delayed when ts-p53TA is expressed. (E) Quantitation of the pattern of BrdU incorporation in HIO107-TA cells after 72 h of growth at 32°C. The majority of the cells are BrdU positive at 39°C, while at 32°C many of the cells are either BrdU negative or have punctate BrdU staining. (F) At 32°C, some HIO107-TA cells exhibit punctate BrdU staining which colocalizes with the telomeric protein TRF2 (arrow). The other two staining patterns also shown are diffuse nuclear staining or no BrdU incorporation.

    Article Snippet: The precleared chromatin was incubated overnight at 4°C with the following primary antibodies: goat polyclonal human TRF2 (Imgenex), goat polyclonal p53 (C-19; Santa Cruz), or goat serum IgG (Santa Cruz).

    Techniques: Expressing, Cell Culture, Generated, Quantitation Assay, BrdU Incorporation Assay, BrdU Staining, Staining

    FIG. 4. ChIP analysis. (A) Telomeric DNA is precipitated by anti- bodies against the telomeric binding proteins TRF2 but not by non- specific goat IgG, irrespective of culture conditions. Immunoprecipi- tation requires formaldehyde cross-linking of proteins to DNA. p53 is also associated with telomeric DNA in the HIO107-TA cell line but only at the permissive temperature of 32°C. Antibodies against TRF2 do not immunoprecipitate centromeric -satellite sequences. (B) Quantitation of the data shown in panel A. Percent input precip- itated (ppted) [(signal strength of telomeric DNA precipitated with the indicated specific antibody background signal obtained with nonspecific IgG)/input signal] 100. (C) Agarose gel of PCR products using primers to amplify the p53 binding site at position 1.4 kb within the p21WAF1 promoter. The template DNAs are identical to those interrogated for the experiment shown in panel C. The predicted product of 113 bp is only detected in DNA from the ChIP assay carried out with an antibody against p53 at 32°C. MW, PhiX HaeIII markers. Antibodies used for all panels are as follows: Gt TRF2, goat polyclonal antibody against TRF2 (Imgenex); Gt p53, goat polyclonal antibody against the carboxy terminus of human p53 (C-19; Santa Cruz); Gt IgG, goat IgG (Santa Cruz).

    Journal: Molecular and Cellular Biology

    Article Title: p53 Differentially Inhibits Cell Growth Depending on the Mechanism of Telomere Maintenance

    doi: 10.1128/mcb.24.13.5967-5977.2004

    Figure Lengend Snippet: FIG. 4. ChIP analysis. (A) Telomeric DNA is precipitated by anti- bodies against the telomeric binding proteins TRF2 but not by non- specific goat IgG, irrespective of culture conditions. Immunoprecipi- tation requires formaldehyde cross-linking of proteins to DNA. p53 is also associated with telomeric DNA in the HIO107-TA cell line but only at the permissive temperature of 32°C. Antibodies against TRF2 do not immunoprecipitate centromeric -satellite sequences. (B) Quantitation of the data shown in panel A. Percent input precip- itated (ppted) [(signal strength of telomeric DNA precipitated with the indicated specific antibody background signal obtained with nonspecific IgG)/input signal] 100. (C) Agarose gel of PCR products using primers to amplify the p53 binding site at position 1.4 kb within the p21WAF1 promoter. The template DNAs are identical to those interrogated for the experiment shown in panel C. The predicted product of 113 bp is only detected in DNA from the ChIP assay carried out with an antibody against p53 at 32°C. MW, PhiX HaeIII markers. Antibodies used for all panels are as follows: Gt TRF2, goat polyclonal antibody against TRF2 (Imgenex); Gt p53, goat polyclonal antibody against the carboxy terminus of human p53 (C-19; Santa Cruz); Gt IgG, goat IgG (Santa Cruz).

    Article Snippet: The precleared chromatin was incubated overnight at 4°C with the following primary antibodies: goat polyclonal human TRF2 (Imgenex), goat polyclonal p53 (C-19; Santa Cruz), or goat serum IgG (Santa Cruz).

    Techniques: Binding Assay, Quantitation Assay, Agarose Gel Electrophoresis